Request QuoteCatalog Number: xP017614TMTSize: 0.2-1mg

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Recombinant Phosphoenolpyruvate carboxykinase [GTP] (pckG)

Recombinant Phosphoenolpyruvate carboxykinase [GTP] (pckG) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP017614TMTYeast1mgQuote
EP017614TMTE. coli1mgQuote
BP017614TMTBaculovirus200ugQuote
MP017614TMTMammalian Cell200ugQuote

Protein Information

SpeciesThermococcus sibiricus (strain MM 739 / DSM 12597)
UniProt IDC6A0S4
Gene NamepckG; Locus:TSIB_0151
Protein NamePhosphoenolpyruvate carboxykinase [GTP]
Region Expressed1-622
Expression Tag6xHis
Purity>90%
AA SequenceMTPEDYLKKRLDPEQFEKIKGIDNPELNEFLAKYIELLNPARVFICTDSKEDENYIRRRA IEYGEEKSLAMEGHTIHYDGYYDQARDKARTKILVPKGVEIPFINTMDREKGLKEIHEIM KDIAKGKELFVCFFVLGPKNSVFTIPAVQLTDSAYVAHSEFILYRKGYEEFKRLGREAKF LKFVHSAGELDERKTSKNIDKRRVYIDLEGETVYSANTQYGGNTIGLKKLAFRLTIKRAV EEGWLSEHMFLMRINGPNGRKTYFTGAYPSMCGKTSTAMISWENIVGDDLTFIVDMKGEA RGANVEKGVFGIIQGVNQEDDPIIWEVLHSPNEIIFSNVLVKDGKPYWNEMGIPIPDEGE NHSGKWWRGKKDSEGNEISPSHKNARFTVSLDAFPNTDLEALETPCGVRVGGMIFGGRDA DTWPPVREAFDWAHGVITMGAALESETTAATLGKEGVRAFNPMAILDFLSVHIGKYLKNY LEFEKKLRIKPKIFAVNYFLREKDGKWLNHKLDKSVWLKWMELRVHGDVDAIKTPVGYIP KYEDLKRLFKEVLNKDYSREDYEKQFVIRVPEFLAKIERIEKIYRDVGNIPEELFKVLEE ERHRLIEAKEKYGDYISPFKFL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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