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Recombinant Phosphatidylcholine-sterol acyltransferase (Lcat)

Recombinant Phosphatidylcholine-sterol acyltransferase (Lcat) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP012783RAYeast1mgQuote
EP012783RAE. coli1mgRPJ516Ra01
BP012783RABaculovirus200ugQuote
MP012783RAMammalian Cell200ugQuote

Protein Information

SpeciesRattus norvegicus (Rat)
UniProt IDP18424
Gene NameLcat
Protein NamePhosphatidylcholine-sterol acyltransferase
Region Expressed25-440
Expression Tag6xHis
Purity>90%
AA SequenceFWLLNVLFPPHTTPKAELSNHTRPVILVPGCMGNRLEAKLDKPNVVNWLCYRKTEDFFTI WLDFNMFLPLGVDCWIDNTRVVYNRSSGHMSNAPGVQIRVPGFGKTYSVEYLDDNKLAGY LNTLVQNLVNNGYVRDETVRAAPYDWRLAPRQQDEYYQKLAGLVEEMYAAYGKPVFLIGH SLGCLHVLHFLLRQPQSWKDHFIDGFISLGAPWGGSIKPMRILASGDNQGIPIMSNIKLR EEQRITTTSPWMFPAHHVWPEDHVFISTPNFNYTGQDFERFFADLHFEEGWHMFLQSRDL LAGLPAPGVEVYCLYGVGMPTAHTYIYDHNFPYKDPVAALYEDGDDTVATRSTELCGQVQ GRQSQGVHLLRMNGTDHLNMVFSNKTLEHINAILLGAYPHGTPKSPTASLGPPPTE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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