Request QuoteCatalog Number: xP524986SXVSize: 0.2-1mg

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Recombinant Phenylalanine--tRNA ligase alpha subunit (SPAC3G9.06)

Recombinant Phenylalanine--tRNA ligase alpha subunit (SPAC3G9.06) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP524986SXVYeast1mgQuote
EP524986SXVE. coli1mgQuote
BP524986SXVBaculovirus200ugQuote
MP524986SXVMammalian Cell200ugQuote

Protein Information

SpeciesSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
UniProt IDO42870
Gene NameORFs:SPAC3G9.06
Protein NamePhenylalanine--tRNA ligase alpha subunit
Region Expressed1-499
Expression Tag6xHis
Purity>90%
AA SequenceMSKLEALQVFLLEKLNEKNEIPNTSHLEFDGKKLGPQEAQSAILSLAAKNMIEFSRHEIE IYNLTAEGENICANGSHEAKVYNEICASMSGLNIGELKKKLGNSAGIGQGRAFKLGWIKK DGDKLVKNTDSITDETPKVLSEIKEHGTISDSKTLTDLKKRKLVERNKIMYFSLRKGPNF SLQIEKLNTDLTAEMITSRSWESAKFKSYNFAAEGIPPAGGCLHPLMKVREEFRKFFFEL GFEEMPTNNFVESGFWNFDALFVPQQHSARDAQDTFFLKVPASTDKLPDPEYVARVKATH ENGGETKGIGYRAPFSLEETRKLVLRTHTTAVSANMLYKLAQNGFHPAKYFSIDRVFRNE TVDATHLAEFHQVEGVICDRNITLGDLIGFLEVFFGKMNVKNLRFKPAYNPYTEPSLEVF SYHEKLGKWVEVGNSGMFRPEMLEPMGLPKDVRCLGFGLSLERPTMIKYGVADIRQLIGP KVNLDLIEASPAVRLDKEE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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