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Recombinant M-phase inducer phosphatase 1 (Cdc25a)

Recombinant M-phase inducer phosphatase 1 (Cdc25a) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP004994RAYeast1mgQuote
EP004994RAE. coli1mgQuote
BP004994RABaculovirus200ugQuote
MP004994RAMammalian Cell200ugQuote

Protein Information

SpeciesRattus norvegicus (Rat)
UniProt IDP48965
Gene NameCdc25a
Protein NameM-phase inducer phosphatase 1
Region Expressed1-525
Expression Tag6xHis
Purity>90%
AA SequenceMELGPEPPHRRRLLFTCSPTPAPQPTGKVQFGASRAGGLSPVTNLTVTMDQLEGLGSDYE KPMDVRNSSSLQRMGSSESTDSGFCLDSPGPLDSKENLEISLRRINCLPQKLLGCSPALK RSHSDSLDHDIFQLIDQDENKENEAFEFKKPIRPASRGCLNAHVHEESKDPFTHRQNSAP ARMLSSNESDISESGNFSPLFTPQSPVKASLSDEDDGFIDLLDGENLKNDEETPSCMSSL WTAPLVMRRPTNLADRCGLFDSPSPCSSTSSCSTRAVKRADRSHEESPRGTKRRKSSEAS PVKADVPEPTQLPHQSLSLTSFPKGTIENIFHSDPRDLIGDFSKGYLFHTVSGKHQDLKY ISPEIMASVLNGKFANLIKEFVIIDCRYPYEYEGGHIKGAVNLHMEEEVEEFLLKKPIVP ADGKRVIVVFHCEFSSERGPRMCRYVRERDRLGNEYPKLHYPELYVLKGGYKEFFLKCQS HCEPPSYRPMHHEDFKEDLKKFRTKSRTWAGEKSKREMYSRLKKL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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