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Recombinant Peptide chain release factor 1 (prfA)

Recombinant Peptide chain release factor 1 (prfA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP015197BYIYeast1mgQuote
EP015197BYIE. coli1mgQuote
BP015197BYIBaculovirus200ugQuote
MP015197BYIMammalian Cell200ugQuote

Protein Information

SpeciesAmoebophilus asiaticus (strain 5a2)
UniProt IDB3ES89
Gene NameprfA; Locus:Aasi_0702
Protein NamePeptide chain release factor 1
Region Expressed1-355
Expression Tag6xHis
Purity>90%
AA SequenceMIDKLEAIEKKYEEVSKQIVDPSIMADMKQYASLNKTYKELSKIVDVYTVYKSNLANLES AKTLLSIEKDAAFRDLAKEEIDSLELQKTQLEEDLKFLLLPKDPNDSKNVILEIRAGTGG DEAGIFAGDLFRMYSRFAEKMQWKLSIIEEVESTSGGYKEIICSIAGEGAYGMLKYESGV HRVQRVPATETQGRIHTSAASVVVLPEMDEVEVDLDMNDIRKDTFCSSGPGGQSVNTTYS AIRLTHIPTGIVVSCQDEKSQIKNLEKALKVLRARLYEQELKKQQDAIGAERRSMVKSGD RSDKIRTYNFPQGRVTDHRIGYTIHNLPAVMDGAVGDLIEALQLADNAERLQQGA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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