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Recombinant Peptide chain release factor 1 (prfA)

Recombinant Peptide chain release factor 1 (prfA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP015197DSQYeast1mgQuote
EP015197DSQE. coli1mgQuote
BP015197DSQBaculovirus200ugQuote
MP015197DSQMammalian Cell200ugQuote

Protein Information

SpeciesChlorobaculum parvum (strain NCIB 8327) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 263 / NCIB 8327) )
UniProt IDB3QRH7
Gene NameprfA; Locus:Cpar_0069
Protein NamePeptide chain release factor 1
Region Expressed1-357
Expression Tag6xHis
Purity>90%
AA SequenceMFDKLQSIKDKYLTIEQQLSDPEVAADQNRFRKLNKEYSGLREIVRAYDNWRSAKQQLDE TLAMQKSEEDPEMRALAAAEAEELQERLPELEQNLKILLLPKDEADTRNAIIEIRAGTGG DEAGLFAADLMRMYQRYAERQGWSCQTLDVSEGSVPGSLKEVSLEVSGHDVYGILKFESG VHRVQRVPETETQGRIHTSAASVAVLPEAEEVDVEIRKDDLQMDTFRSGGKGGQNVNKVE TAVRITHVPSGIVVACQEERSQLQNRERAMKMLRSKLYDIKITEQLQSRADLRRSMVATG DRSAKIRTYNFPQSRVTDHRIGFTTHALPQVMQGELEPIIDALRMHDQAERLKAETN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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