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Recombinant Peptide chain release factor 1 (prfA)

Recombinant Peptide chain release factor 1 (prfA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP015197DZSYeast1mgQuote
EP015197DZSE. coli1mgQuote
BP015197DZSBaculovirus200ugQuote
MP015197DZSMammalian Cell200ugQuote

Protein Information

SpeciesCupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424) )
UniProt IDB3R722
Gene NameprfA; Locus:RALTA_A2795
Protein NamePeptide chain release factor 1
Region Expressed1-360
Expression Tag6xHis
Purity>90%
AA SequenceMKASMLAKLDQLAERLDEVNALLAREDATANIDQYRKLSREHAELSPVAEQYSQYRQAQD DLATAQALLDDPEMKDFAADEIAAARDRLEALQASLQRLLLPKDPNDDRNLLLEIRAGTG GEESALFAADLLRMYTRYAERQRWQVEVMSESESDLGGYKEVIIRIAGDAAFSRLKFESG GHRVQRVPATEAQGRIHTSACTVAVMPEADEVGEVEINPADLRIDTFRASGAGGQHVNKT DSAVRLTHLPTGIVVECQDDRSQHRNKDKAMKVLAARIKDMQTRAAQAREASTRRNLIGS GDRSDRIRTYNFPQGRVTDHRINLTLYKIDMIMDGDMDELLSALSAEHQADQLAALGEDA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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