Platelet-Derived Growth Factor AB Human Recombinant
Platelet-Derived Growth Factor AB Human Recombinant is a heterodimeric, non-glycosylated, polypeptide chain containing 234 amino acids consisting of 13.3kDa alpha-chain and 12.2 beta-chain having a total molecular mass of 25.5kDa.
PDGF-AB is purified by proprietary chromatographic techniques.
Accession
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Ile-Glu-Glu-alpha chain and Met-Ser-Leu-Gly-Ser-beta chain.
Biological Activity
The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is < 1 ng/ml, corresponding to a Specific Activity of 1 MIU/mg.
Formulation
The protein was lyophilized with no additives.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-AB in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Stability
Lyophilized Platelet-derived Growth Factor AB although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution PDGF-AB should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AB.
