$90.00Catalog Number: PYT590-2ugSize: 2ug

PDGF AA Mouse

Platelet-derived Growth Factor AA Mouse Recombinant

PDGF-AA Mouse Recombinant is a disulfide linked homodimeric, non-glycosylated, polypeptide chain containing 2 x 126 amino acids and having a total molecular mass of 28.9 kDa.
PDGF-AA is purified by proprietary chromatographic techniques.

Accession

P20033

Amino acid sequence

MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETGR RRESGKNRKR KRLKPT.

Biological Activity

Established by the dose-dependent stimulation of Balb/c 3T3 cells proliferation. The expected ED50  for this effect is 8-10 ng/ml corresponding to a specific activity of 100,000-125,000IU/mg.

Formulation

The protein was lyophilized with no additives.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized PDGF-AA in sterile 18MΩ-cm H2O at a concentration ranging between 0.1-0.5mg per 1ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized PDGF-AA although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution PDGF-AA should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1.

Quantity

$90.00

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