PEST proteolytic Signal Containing Nuclear Protein Human Recombinant
PCNP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 202 amino acids (1-178 a.a.) and having a molecular mass of 21.5kDa. PCNP is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Accession
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSHMADGKA GDEKPEKSQR AGAAGGPEEE AEKPVKTKTV SSSNGGESSS RSAEKRSAEE EAADLPTKPT KISKFGFAIG SQTTKKASAI SIKLGSSKPK ETVPTLAPKT LSVAAAFNED EDSEPEEMPP EAKMRMKNIG RDTPTSAGPN SFNKGKHGFS DNQKLWERNI KSHLGNVHDQ DN.
Formulation
PCNP protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 1mM DTT.
Physical Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Source
Stability
Store at 4oC if entire vial will be used within 2-4 weeks. Store, frozen at -20oC for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Synonyms
PEST proteolytic signal-containing nuclear protein, PCNP, PEST-containing nuclear protein.
