$75.00Catalog Number: PTN303-5ugSize: 5ug

PCNA Human, Sf9

Proliferating Cell Antigen Human Recombinant, Sf9

PCNA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 29,794 Dalton.
PCNA is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Accession

P12004

Applications

Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.

Coating concentration

0.5-1.1 ug/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.

Formulation

Recombinant Human PCNA is supplied in16mM HEPES buffer pH-8, 400mM NaCl, and 20% glycerol.

Immunological Function

1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot analysis with validated SLE patient sera.

Physical Appearance

Sterile Filtered colorless solution.

Purity

Greater than 95% as determined by SDS-PAGE.

Source

Sf9 insect cells.

Storage

Store at 4oC if entire vial will be used within 2-4 weeks.
Store, frozen at -20oC for longer periods of time.
Avoid multiple freeze-thaw cycles.

Synonyms

Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367.

Quantity

$75.00

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