PCNA antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 3,000. Recommended starting dilution is 1:2,000.
Mouse IgG3 heavy chain and κ light chain.
Anti-human PCNA mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PCNA amino acids 1-261 purified from E. coli.
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
PCNA antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
12 months at -20oC. 1 month at 4oC.
For periods up to 1 month store at 4oC, for longer periods of time, store at -20oC. Prevent freeze thaw cycles.
Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367.
Mouse Anti Human Monoclonal.