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Recombinant 2-oxoisovalerate dehydrogenase subunit alpha (bkdA1)

Recombinant 2-oxoisovalerate dehydrogenase subunit alpha (bkdA1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP864221EZXYeast1mgQuote
EP864221EZXE. coli1mgQuote
BP864221EZXBaculovirus200ugQuote
MP864221EZXMammalian Cell200ugQuote

Protein Information

SpeciesPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
UniProt IDQ9I1M2
Gene NamebkdA1; Locus:PA2247
Protein Name2-oxoisovalerate dehydrogenase subunit alpha
Region Expressed1-410
Expression Tag6xHis
Purity>90%
AA SequenceMSDYEPLRLHVPEPTGRPGCKTDFSYLHLSPAGEVRKPPVDVEPAETSDLAYSLVRVLDD DGHAVGPWNPQLSNEQLLRGMRAMLKTRLFDARMLTAQRQKKLSFYMQCLGEEAIATAHT LALRDGDMCFPTYRQQGILITREYPLVDMICQLLSNEADPLKGRQLPIMYSSKEAGFFSI SGNLATQFIQAVGWGMASAIKGDTRIASAWIGDGATAESDFHTALTFAHVYRAPVILNVV NNQWAISTFQAIAGGEGTTFANRGVGCGIASLRVDGNDFLAVYAASEWAAERARRNLGPS LIEWVTYRAGPHSTSDDPSKYRPADDWTNFPLGDPIARLKRHMIGLGIWSEEQHEATHKA LEAEVLAAQKQAESHGTLIDGRVPSAASMFEDVYAELPEHLRRQRQELGV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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