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Recombinant 2-oxoglutarate dehydrogenase E1 component (odhA)

Recombinant 2-oxoglutarate dehydrogenase E1 component (odhA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP504696EPUYeast1mgQuote
EP504696EPUE. coli1mgQuote
BP504696EPUBaculovirus200ugQuote
MP504696EPUMammalian Cell200ugQuote

Protein Information

SpeciesExiguobacterium sp. (strain ATCC BAA-1283 / AT1b)
UniProt IDC4L3W2
Gene NameodhA; Locus:EAT1b_2538
Protein Name2-oxoglutarate dehydrogenase E1 component
Region Expressed1-951
Expression Tag6xHis
Purity>90%
AA SequenceMGESSNVHEKSQAFYGPNLGYIIELYEQYLEDPSSVDEETRRYFDEFGAPETSTPTEVPV SGPSFDLEKVVSATRLINDIRAFGHKAADIYPLKDHPREQELFELSRYDLTEEDLKQIPA RLLSDDAPKPNASALELFRHLLDVYTGTVAIELRHLDDMEEKKWIRRQVEQGALQQTFSK EEKIELFKRLAETELFESFLHKTYVGQKRFSIEGLDAMVPLLDAMVGGLISSGSEHINIG MAHRGRLNVLAHVLGKPYEMIFAEFQHAPNKELIPSEGSIGINFGWSGDVKYHLGLDRKV VEQQKEVRLNLANNPSHLEFVGSVVEGYTRAAQDDRSEKGSAVQHDDLAASILIHGDAAF PGQGIVAETLNMTNLTGYRTGGTVHVIANNTIGFTTDPNDSRSTRYASDIAKGYEIPVFH VNADDPEACVAVAKLISEYRAKFHKDILVDLIGYRRYGHNEMDEPMNTNPVLYKAIKGHQ SVRHVYAARLEEEGVMTKDEKAQIEQKIEEALKAARDLVPSEEEDADIVLPDAVHKGFPK VDTSVEREFLTQLNEELLNWPEGFGVFHKLQKVLDRRRDAFSEGGKIDWGHAETLAFASI LSDGTPVRLSGQDSERGTFAQRNIMLNDVESGKKFSPLHELSTAKASFSVYNSPLSEGSV LGFEYGYNVFAQDTLVLWEAQYGDFANSAQVMFDQFISAGRAKWGQKSGLVMLLPHGYEG QGPEHSSARMERYLTLAGEKNWTVANLSSAAQYFHILRRQAEMLGKEEIRPMIIMTPKSL LRHPLATSPVEAFTEESFKPIVEQPGLGENTEKVERLVFCTGKMAIDLAEAVGKSEESLD FLHIVRVEEIYPFPVREIRDVISRYPNAREIVWVQEEPKNMGAWTYIEPRLEAVTTNRLD VRYIGRRRRSSPAEGNPTAHKQEQARIIREALSRDVVSSGAGTSTYQKDRK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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