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Recombinant 3-oxo-5-beta-steroid 4-dehydrogenase (Akr1d1)

Recombinant 3-oxo-5-beta-steroid 4-dehydrogenase (Akr1d1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001548RAYeast1mgQuote
EP001548RAE. coli1mgQuote
BP001548RABaculovirus200ugQuote
MP001548RAMammalian Cell200ugQuote

Protein Information

SpeciesRattus norvegicus (Rat)
UniProt IDP31210
Gene NameAkr1d1
Protein Name3-oxo-5-beta-steroid 4-dehydrogenase
Region Expressed1-326
Expression Tag6xHis
Purity>90%
AA SequenceMNLSTANHHIPLNDGNSIPIIGLGTYSDPRPVPGKTFIAVKTAIDEGYRHIDGAYVYRNE HEVGEAIREKVAEGKVKREEIFYCGKLWSTDHDPEMVRPALERTLQTLKLDYIDLYIIEM PMAFKPGEEFYPKDENGRVIYHKSNLCATWEALEACKDAGLVKSLGVSNFNRRQLEVILN KPGLKYKPVTNQVECHPYFTQTKLLEVSASSMTSFIVAYSPLGTCRNPLWVNVSSPPLLK DELLTSLGKKYNKTQAQIVLRFDIQRGLVVIPKSTTPERIKENFQIFDFSLTKEEMKDIE ALNKNVRFVEMLMWSDHPEYPFHDEY
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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