Request QuoteCatalog Number: xP340689DPSSize: 0.2-1mg

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Recombinant Nitrogenase molybdenum-iron protein beta chain (nifK)

Recombinant Nitrogenase molybdenum-iron protein beta chain (nifK) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP340689DPSYeast1mgQuote
EP340689DPSE. coli1mgQuote
BP340689DPSBaculovirus200ugQuote
MP340689DPSMammalian Cell200ugQuote

Protein Information

SpeciesAzospirillum brasilense
UniProt IDP25314
Gene NamenifK
Protein NameNitrogenase molybdenum-iron protein beta chain
Region Expressed1-521
Expression Tag6xHis
Purity>90%
AA SequenceMSMSHPVSQSADKVIDHFTLFRQPEYKELFERKKTEFEYGPTPTKEVARVSAWTKTEEYK EKNLPVEAVVINPTKACQPIGAMLAAQGFEGTLPFVHGSQGCVSYYRTHLTRHFKEPNSA VSSSMTEDAAVFGGLNNMIDGLQRYALYKPKMIAVLTTCMAEVIGDDLSGFINNAKNKES VPADFPVPFAHTPAFVGSHIVGYDNMIKGVLTHFWGTSENFDTPKNETINLIPGFDGFAV GNNRELKRIAGLFGIQMTILSDVSDNFDTPADGEYRMYDGGTPLEATKEAVHAKATISMQ EYCTPQSLQFIKREGPAGRQAYNYPMGVTGTDELLMKLAELSGKPSRGVKLERGRLVDAI ADSHTHLHGKRFAVYGDPDFCLGMSKFLMELGAEPVHILSTSGSKKWEKQVQKVLDACRS ASSGKAYGAKDLWHLRSLVFTDKVDYIIGNSYGKYLERDTKIPLIRLTYPIFDRHHHHRY PTWGYQGALNVLVRILDRIFEDMDANTNIVGETDYSFDLVR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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