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Recombinant Nicotinate phosphoribosyltransferase (pncB)

Recombinant Nicotinate phosphoribosyltransferase (pncB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP015451DJKYeast1mgQuote
EP015451DJKE. coli1mgQuote
BP015451DJKBaculovirus200ugQuote
MP015451DJKMammalian Cell200ugQuote

Protein Information

SpeciesDesulfobacterium autotrophicum (strain ATCC 43914 / DSM 3382 / HRM2)
UniProt IDC0QFM5
Gene NamepncB; Locus:HRM2_02990
Protein NameNicotinate phosphoribosyltransferase
Region Expressed1-389
Expression Tag6xHis
Purity>90%
AA SequenceMIQSILDNDLYKFTMQQAVHMLYPRVDVEYEFINRSNTPFPKDFAQRLQVEVQGMKNFRL TPEEKEYLDKTCYFMTPVYLDFLEHYTFDPDEVTVSQTNSELSVTIKGPWYRTILWEVPL MAIISELYFVMTNARPLPDEQIRVINLNKAKILSCNNIRYADFGTRRRFSSSGHEALIRD ILALEHNTLIGTSNVNLARLFNIKPIGTMAHEWIMFHGVLNGYRMANPTAVAAWATAFHG HLGIALTDTFTTDIFLSTFDTLHAKLFDGVRHDSGDPIAFIDRIVDHYKKLHIDPITKTI VFSDGLDIDKAVHIHNHCINRIRDSYGIGTNLTNDVGVTPLNMVIKLAKCRTAPEKDWHN AIKLSDDKGKHTGDSEELAHCIKVLERGM
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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