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Recombinant Neutral trehalase (treB)

Recombinant Neutral trehalase (treB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP520373NHAYeast1mgQuote
EP520373NHAE. coli1mgQuote
BP520373NHABaculovirus200ugQuote
MP520373NHAMammalian Cell200ugQuote

Protein Information

SpeciesNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
UniProt IDO42783
Gene NametreB; ORFs:G15D1.050, NCU04221
Protein NameNeutral trehalase
Region Expressed1-744
Expression Tag6xHis
Purity>90%
AA SequenceMTSQPSSGKGRGRNLSIDEYNVYDDAKTYYTTEDRHHNHRAGARTRTYSQNSLFKQFERL GLQKEPYRRGSHDESTIPQSRRFLIQVEPTLQSLQSQEDTDGNMQITIEDNGPKVLSLRT AASNGYNRFDIRGTYMLSNLLQELYLAKEYGRKQIILDEARLNENPVNRLSRLIKDHFWE GLTRRIDASSIEIAARDPKDWTDDPRPRIYIPRGAPEQHEYYTKVALDRPELRLDVQYLP EKITPEIVRDMNAKPGLLAVDMEEVVDPKTGEKTLRGRPFVVPGGRFNELYGWDSYMESL GLLVNDRVDLAKSMVQNFCFCIKHYGKILNATRSYYLCRSQPPFLTDMTLRVYDKIKHEP GALEFLRQSLLAAIKEYYSVWTAEPRLDPVTGLSRYRPEGLGVPPETEAGHFIHILEPYA KKHNMSFDEFVYAYNHGEIKEPTLDDYFMHDRAVRESGHDTTYRFEGICADLATIDLNSL LFKYETDIARTIRNVFHDKFEVPDDWLATNNPAASKLETSAMWDRRAKRRKLAIDKYLWN EEAGMYFDYNTATRKQCNYESATTFWALWAGVSNPKQAAAMVTKALPKLEAFGGLLSGTK ESRGEIGLERPNRQWDYPYGWAPQQILAWTGLYRYGFNEEAERLAYKWLFMITKAFVDFN GVVVEKYDVTRPIDPHRVDAEYGNQGLDFKGVAKEGFGWVNASYVYGLQIVNAHMRRALG TLTPYETFMKAVEENRNKALSELV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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