Request QuoteCatalog Number: xP527315SXVSize: 0.2-1mg

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Recombinant NEDD8-specific protease 1 (nep1)

Recombinant NEDD8-specific protease 1 (nep1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP527315SXVYeast1mgQuote
EP527315SXVE. coli1mgQuote
BP527315SXVBaculovirus200ugQuote
MP527315SXVMammalian Cell200ugQuote

Protein Information

SpeciesSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
UniProt IDO42980
Gene Namenep1; ORFs:SPBC17D11.01, SPBC20F10.11
Protein NameNEDD8-specific protease 1
Region Expressed1-420
Expression Tag6xHis
Purity>90%
AA SequenceMSSSPTVLELFDVCFKQEDVDSLKKPNWFTDVSIDYVDELIEHLWFPSYPNQANEILLLR PSLVFLLAEAAISPEELKVALPKKLMNCKYLFMPINDLDKHAAGSGGSHWSLMVASIPDG QCYYYDSLSNGKTKDCRSALARVSDLFKKKFTIECMPVQQQRNGYDCGAHVCAFTLELVR RLLHSPMPTSSMWNLSTFQPDVTAIREQLSRCLDHIINSLGTRVSGDFDEDFPTGTVFFD LESHLPLLDVALPVLPKSSDSSETSHESSNSNLKKSSESGSTNHHNNHESDKDLHHEGHH HHHHHHHHHHSHDDDPSSPAEKKQNHVPSPSEKIQDHVPSPSEKKQDRVPSPSNNKEDHL PLLSDEKLDKSAIDKIEPTPLPSVHMNSHIAKGELPKFHNSTDNPFLTPPEELVSGDFPF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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