NANOG antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500~1:1,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:500 and Immunohistochemistry is 1:50.

P5A10AT.

Mouse IgG2a heavy chain and κ light chain.

Anti-human NANOG mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human NANOG amino acids 1-154 purified from E. coli.

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

NANOG antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

12 months at -20^oC. 1 month at 4^oC.

For periods up to 1 month store at 4^oC, for longer periods of time, store at -20^oC. Prevent freeze thaw cycles.

NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog.

Mouse Anti Human Monoclonal.