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Recombinant Modification methylase Eco57IB (eco57IBM)

Recombinant Modification methylase Eco57IB (eco57IBM) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP340682ENLYeast1mgQuote
EP340682ENLE. coli1mgQuote
BP340682ENLBaculovirus200ugQuote
MP340682ENLMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli
UniProt IDP25240
Gene Nameeco57IBM; aka: eco57IM
Protein NameModification methylase Eco57IB
Region Expressed1-544
Expression Tag6xHis
Purity>90%
AA SequenceMKFKADQTSQKLRGGYYTPQNLADYVTKWVLSKNPKTILEPSCGDGVFIQAIANNGYNSN IELFCFELFDTEASKALERCKLNNFSNATITEGDFLVWANECLKKNKQIFDGALGNPPFI RYQFLERNFQEQAQLVFEHLDLKFTKHTNAWVPFLLSSLALLKQGGRIGMVIPSEISHVM HAQSLRSYLGHVCSKIVIIDPKEIWFEDTLQGAVILLAEKKQYPDEASQGVGIVSVSGFE FLQEDPNVLFNDTAGINGETVEGKWTKATLSIDELQLIKRVIAHPDVRKFKDIAKVDVGR YCDGANNYFLVDNETVKLYKLERFAHPMFGRSQHCPGIIYDEKQHIENQEKGLPTNFLYI DEEFEYLSKSVKNYIKLGEVEEYHKRYKCRIRKPWFKVPSVYSTEIGMLKRCHDAPRLIH NRVRAYTTDTAYRVSSTVTSTENLVCSFLNPITVITAELEGLFYGGGVLELVPSEIEKLY ILIVEGLEHNVEELNLLIKDGQIERVIRQQGSLILGTLGFTQEENEKLVEIGRSLEIEGY VSRV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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