$148.20Catalog Number: PYT596-10ugSize: 10ug

MIF Human, Active

Macrophage Migration Inhibitory Factor Human Recombinant (Active)

MIF human Recombinant was cloned into an E.coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.
Macrophage Inducing Factor Human Recombinant is a single, non-glycosylated, polypeptide chain containing 115 amino acids and having a molecular mass of 12.5 kDa.

Accession

P14174

Amino Acid Sequence

MPMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLM
AFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVY
INYYDMNAANVGWNNSTFA.

Biological Activity

Human MIF biological activity was measured in an agarose microdroplet assay usinghuman U937 cells astargets.The optimal range of activity found for human MIF in this assay was 500-1,000ng/ml corresponding to a specific activity of 1,000-2,000IU/mg.

Formulation

MIF-Protein was lyophilized from 10mM sodium phosphate buffer pH-7.5.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized MIF-Protein in sterile 18MΩ-cm H2O at a concentration between 0.1mg-1mg per 1ml.

Source

Escherichia Coli.

Stability

Lyophilized MIF-protein although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution MIF-protein should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, MMIF, MIF.

Quantity

$148.20

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