$90.00Catalog Number: PYT310-5ugSize: 5ug

MIA Human

Melanoma Inhibitory Activity Human Recombinant

Melanoma Inhibitory Activity Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain consisting of 108 amino having a total molecular mass of 12237 Dalton.
The MIA is purified by proprietary chromatographic techniques.

Accession

Q16674

Amino acid sequence

MGPMPKLADRKLCADQECSSHPISMAVALQDYMAPDCRFLTIHRGQVV
YVFSLKGRGRFLWGGSVQGDYYGDLAARLGYFPSSIVREDQTLKVDVKT
DKWDFYCQ
Note: The N-terminal methionine is not present in natural MIA.

Biological Activity

The biological activity is calculated by the inhibiting effect on the invasion of Mel In Tumor cells and found active in Mel In assay.

Formulation

The protein was lyophilized from a concentrated (1.66mg/ml) solution containing 20mM Potassium-phosphate pH=7 and 150mM potassium chloride.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content

UV spectroscopy at 280 nm using the absorption coefficient of 19300 M-1cm-1 .

Purity

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Melanoma Inhibitory Activity in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized MIA although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution MIA should be stored at 4oC between 2-7 days and for future use below
-18oC.
Please prevent freeze-thaw cycles.

Synonyms

Melanoma-derived growth regulatory protein precursor, Cartilage-derived retinoic acid-sensitive protein, CD-RAP, MIA.

Quantity

$90.00

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