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Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)

Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP759442PIJYeast1mgQuote
EP759442PIJE. coli1mgQuote
BP759442PIJBaculovirus200ugQuote
MP759442PIJMammalian Cell200ugQuote

Protein Information

SpeciesPhotorhabdus luminescens subsp. laumondii (strain TT01)
UniProt IDQ7N8K7
Gene NameispD; Locus:plu0713
Protein Name2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Region Expressed1-243
Expression Tag6xHis
Purity>90%
AA SequenceMNNLLLRSSADIIALVPAAGIGSRMNSDCPKQYLSIAGKTIIEHTLSALLDHPRIRHVVI VLNPTDTQFQSLEVVSDSRITTVTGGEQRADSVLAGLNHLAHVTGNDNCWVLVHDAARPC LHHDDLDRLLQLAEADEQGNMACGGILASPVRDTMKRGRVGQIIDHTVERQDLWHALTPQ FFPLMLLRDCLSKALSQHANITDEASALEYCGYQPVLVNGRSDNIKVTCPEDLALAEFYL SRK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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