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Recombinant 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)

Recombinant 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP754724Yeast1mgQuote
EP754724E. coli1mgQuote
BP754724Baculovirus200ugQuote
MP754724Mammalian Cell200ugQuote

Protein Information

SpeciesListeria monocytogenes serotype 4b (strain F2365)
UniProt IDQ724H6
Gene NameispF; Locus:LMOf2365_0248
Protein Name2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Region Expressed1-157
Expression Tag6xHis
Purity>90%
AA SequenceMIRIGQGYDVHKLAYDRELIVGGIKIPYEKGLLGHSDADVLLHAITDAIIGAIGAGDIGH FFPDTDMAFKDADSAELLEEIWQKVEADGFRLGNLDATIIAEKPKMAPYVEQMKLRIAEL LHADSAQVNVKATTTEKLGFTGREEGIASLAVVLLEK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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