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Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)

Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP350087EZXYeast1mgQuote
EP350087EZXE. coli1mgQuote
BP350087EZXBaculovirus200ugQuote
MP350087EZXMammalian Cell200ugQuote

Protein Information

SpeciesPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
UniProt IDP57707
Gene NameispD; Locus:PA3633
Protein Name2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Region Expressed1-234
Expression Tag6xHis
Purity>90%
AA SequenceMTTSDLPAFWTVIPAAGVGSRMRADRPKQYLDLAGRTVIERTLDCFLEHPMLRGLVVCLA EDDPYWPGLDCAASRHVQRAAGGAERAGSVLNGLLRLLELGAQADDWVLVHDAARPNLTR GDLDRLLEELAEDPVGGLLAVPARDTLKRSDRDGRVSETIDRSVVWLAYTPQMFRLGALH RALADALVAGVAITDEASAMEWAGYAPKLVEGRADNLKITTPEDLLRLQRSFPH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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