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Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)

Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP509658ESQYeast1mgQuote
EP509658ESQE. coli1mgQuote
BP509658ESQBaculovirus200ugQuote
MP509658ESQMammalian Cell200ugQuote

Protein Information

SpeciesPectobacterium carotovorum subsp. carotovorum (strain PC1)
UniProt IDC6DDG2
Gene NameispD; Locus:PC1_3353
Protein Name2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Region Expressed1-237
Expression Tag6xHis
Purity>90%
AA SequenceMQTPSLSPPDIVAVLPAAGNGSRMQNDRPKQYLTIGNDATGHKTILEHTIDALLRHSRVR RVVVVISPDDAFFHTLAIASDPRICTVTGGRQRADSVLAGLSAVADGAWALVHDAARPCL HQDDLTRLLAIVEQSDVGGILAAPVRDTMKRSTDGFIDRTVERNDLWHALTPQLFPAALL KQCLQRALQDGVAVTDEASALEYCGYRPQIISGRADNIKVTRPEDLALAEFYLTSLQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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