Request QuoteCatalog Number: xP500231DUHSize: 0.2-1mg

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Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)

Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP500231DUHYeast1mgQuote
EP500231DUHE. coli1mgQuote
BP500231DUHBaculovirus200ugQuote
MP500231DUHMammalian Cell200ugQuote

Protein Information

SpeciesClostridium botulinum (strain Kyoto / Type A2)
UniProt IDC1FMX6
Gene NameispD; Locus:CLM_3975
Protein Name2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Region Expressed1-229
Expression Tag6xHis
Purity>90%
AA SequenceMSKNSVIIVAAGKGKRMNSSISKQFLQIKNKPILYYTLSKFSTHESIDEIVLVTLEDKIE VCGDIIDKYNINKVSKIVPGGKERQDSVYNGLKAVSKDCEVVLIHDAARPFVTSDIIENG IRYTNQYGAAACGVIPKDTIKIKNEKGFAIDTPNREDLFIVQTPQCFNYNIILSCHEKLK KHNKKVTDDTMVLENYGKSVYLYEGSYSNIKITTPEDLILGEQILEKLT
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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