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Recombinant 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)

Recombinant 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP505708SWUYeast1mgQuote
EP505708SWUE. coli1mgQuote
BP505708SWUBaculovirus200ugQuote
MP505708SWUMammalian Cell200ugQuote

Protein Information

SpeciesSalmonella paratyphi C (strain RKS4594)
UniProt IDC0PXA6
Gene NameispF; Locus:SPC_2972
Protein Name2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Region Expressed1-159
Expression Tag6xHis
Purity>90%
AA SequenceMRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHALTDALLGAAALGDIGKL FPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQAPKMLPHIPQMRVFIAEDL GCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLMKAAK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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