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Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)

Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP458378BXTYeast1mgQuote
EP458378BXTE. coli1mgQuote
BP458378BXTBaculovirus200ugQuote
MP458378BXTMammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia phymatum (strain DSM 17167 / STM815)
UniProt IDB2JGK2
Gene NameispD; Locus:Bphy_0998
Protein Name
Region Expressed1-236
Expression Tag6xHis
Purity>90%
AA SequenceMTSRLFALIPCAGTGSRSGAPMPKQYRTVAGRDMLHYSLAAFDACSEFAQTLVVIAPDDQ HFDARRFGGLRFAVQRCGGASRQASVLNGLHALAGFGAHDDDWVLVHDAARPGITPALIR ALVGALKDDAVGGIMALPVADTLKRVAPGTDNRIDHTEPRDGLWQAQTPQMFRIGMLRDA ILRAQSDGHDLTDEASAIEWSGHAPRLVQGSLRNFKVTYPEDFDLAEAILGRGAAG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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