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Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)

Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP460609BXUYeast1mgQuote
EP460609BXUE. coli1mgQuote
BP460609BXUBaculovirus200ugQuote
MP460609BXUMammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia phytofirmans (strain DSM 17436 / PsJN)
UniProt IDB2T3X2
Gene NameispD; Locus:Bphyt_1875
Protein Name
Region Expressed1-238
Expression Tag6xHis
Purity>90%
AA SequenceMTSRLFALIPCAGTGSRSGAAMPKQYRTVAGRDMLHYSLAAFDACSEFAQTLVVIAPDDA HFDGRRFGGLRFAVRRSGGASRQASVLNGLHALAEFGARDDDWVLVHDAARPGITPALIR TLIGALKDDPVGGIMALPVADTLKRIDATSSDGRIARTEARDGLWQAQTPQMFRIGMLRE AILRAQADGHDLTDEASAIEWLGHAPKLVQGSLRNFKVTYPEDFDLAEAILSRPAAAS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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