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Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)

Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP414778CWWYeast1mgQuote
EP414778CWWE. coli1mgQuote
BP414778CWWBaculovirus200ugQuote
MP414778CWWMammalian Cell200ugQuote

Protein Information

SpeciesClostridium botulinum (strain Langeland / NCTC 10281 / Type F)
UniProt IDA7GJ99
Gene NameispD; Locus:CLI_3691
Protein Name2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Region Expressed1-229
Expression Tag6xHis
Purity>90%
AA SequenceMSKNSVIIVAAGKGKRMNSSISKQFLQIKNKPILYYTLSKFSAHESIDEIVLVTLEDKIE VCSDIIDKYNINKVSKIVPGGKERQDSVYNGLKAVSKDCEVVLIHDAARPFVTSDIIENG IRCANQYGAAACGVIPKDTIKIKNEKEFAIDTPNREDLFIVQTPQCFNYNIILDCHEKLK KHNKKVTDDTMVLENYGKSVYLYEGSYSNIKITTPEDLILGEQILEKLT
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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