The malE gene is inserted in a pMAL vector (Maina et al., 1988) and then expressed in E. coli JM109.
The purified recombinant MBP protein was then used for mouse immunization.

Immunofluorescence: Typical working dilution on frozen sections 1:200. 60 minutes primary antibody incubates at 25^oC.

Mouse monoclonal antibody against the amino acid stretch of KDPRIAA (the amino acid residue 339 to 345) of the periplasmic maltose-binding protein (MBP) [Escherichia coli] (Accession #: AAB59056).

Store unopened antibody at 4^oC or lower. Under these conditions, there is no significant loss in product performance one year from the purchase date. The reconstituted antibody is stable for at least two months when stored at 4^oC. For long term storage, it is recommended that aliquots of the antibody are frozen at -20^oC (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use.

Theammonium sulfate-precipitated ascites fluid is in PBS + 1% BSA & then lyophilized.

Mouse Antibody Monoclonal.

Recommended: working dilution on Western bloting 1:500. 60 minutes primary antibody incubates at 25^oC.