$285.00Catalog Number: MAB177-20ugSize: 20ug

MBP Antibody

Maltose-Binding protein, Mouse Antibody
Monoclonal Antibody

Monoclonal antibodies provide the best target specificity and affinity. It is also advantagous for long term projects as there is little lot to lot variation, where polycolonal antibodies will vary significantly with different preparations due to immunogen preparation and animal responses.

Immunogen

The malE gene is inserted in a pMAL vector (Maina et al., 1988) and then expressed in E. coli JM109.
The purified recombinant MBP protein was then used for mouse immunization.

Recommendations on use

Immunofluorescence: Typical working dilution on frozen sections 1:200. 60 minutes primary antibody incubates at 25oC.

Specificity

Mouse monoclonal antibody against the amino acid stretch of KDPRIAA (the amino acid residue 339 to 345) of the periplasmic maltose-binding protein (MBP) [Escherichia coli] (Accession #: AAB59056).

Storage and Stability

Store unopened antibody at 4oC or lower. Under these conditions, there is no significant loss in product performance one year from the purchase date. The reconstituted antibody is stable for at least two months when stored at 4oC. For long term storage, it is recommended that aliquots of the antibody are frozen at -20oC (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use.

Storage Buffer

Theammonium sulfate-precipitated ascites fluid is in PBS + 1% BSA & then lyophilized.

Type

Mouse Antibody Monoclonal.

Westren Blotting

Recommended: working dilution on Western bloting 1:500. 60 minutes primary antibody incubates at 25oC.

Quantity

$285.00

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