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Recombinant Mannose-6-phosphate isomerase (pmi40)

Recombinant Mannose-6-phosphate isomerase (pmi40) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP014754SXVYeast1mgQuote
EP014754SXVE. coli1mgQuote
BP014754SXVBaculovirus200ugQuote
MP014754SXVMammalian Cell200ugQuote

Protein Information

SpeciesSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
UniProt IDO43014
Gene Namepmi40; ORFs:SPBC2G2.16
Protein NameMannose-6-phosphate isomerase
Region Expressed1-412
Expression Tag6xHis
Purity>90%
AA SequenceMYRLKCDVKSYGWGKVGHESLAAKLAEAGYGFEVDPNTPYAELWMGSHPSGPSFVMQTGK PLSELLTPETVGEKVYKKYGKQLPFLFKVLSINKVLSIQAHPDKPLGKQLHKTNPKEYKD DNHKPEMAVALTEFDALCGFRPVKQIEQFLDSIAPLREFVGEEAVRQFKGTVKQDERKAL ETLFNELMHKDEKRIQEFTPQLVQLAKSDANNFGGTEYGGKAFSDLIIHLNSQFPDDIGL FVTPFLNYVRLHPGEAVFLRALDPHAYVSGNIIECMAASDNVIRLGFTPKFKDIETLVNN LTYQTADAKSQLTQPVPFAKACGSGKTLLYDPPIEEFSILQTKVSPGQKQCIRGINGPSI LLVTEGSGILNGDKGDVASISPGFVYFISANFPLTISATSEPVVVYQAFCEI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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