Leptin Receptor Human Recombinant
Leptin Binding Domain Human Recombinant also called Leptin soluble receptor produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 208 amino acids and having a molecular mass of 24.5 kDa. Leptin Receptor consists of the cytokine binding domain of leptin receptor amino acids 428-635 of human leptin receptor.
The Leptin Binding Domain is purified by proprietary chromatographic techniques.
Accession
Amino acid sequence
The sequence of the first four N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val
Biological Activity
Biological Activity is evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio.
Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Protein content
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 2.45 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Leptin Binding Domain as a Reference Standard.
Purity
Greater than 98.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
The lyophilized Leptin Binding Domain is very soluble in water and most aqueous buffers below and above the isoelectric point.
Source
Stability
Lyophilized Leptin Binding Domain although stable at room temperature, should be stored desiccated below 0oC. Reconstituted Leptin is best stored refrigerated at 4oC.
Please prevent freeze-thaw cycles.
Synonyms
OB Protein, Obesity Protein, OBS, Obesity factor, Leptin Receptor.
