1. Positive control for Western blot.
2. Antibody production.
3. Protein assay.

The protein was lyophilized from a concentrated (1mg/ml) solution with 1X PBS, 0.1% SDS and 1mM DTT.

Sterile Filtered White lyophilized (freeze-dried) powder.

Greater than 90.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

The lyophilized Leptin is very soluble in water and most aqueous buffers below and above the isoelectric point.

Escherichia Coli.

Lyophilized Leptin although stable at room temperature, should be stored desiccated below 0^oC. Reconstituted Leptin is best stored refrigerated at 4^oC.
Please avoid freeze-thaw cycles.

OB Protein, Obesity Protein, OBS, Obesity factor.