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Recombinant Large T antigen

Recombinant Large T antigen can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP340657BKRYeast1mgQuote
EP340657BKRE. coli1mgQuote
BP340657BKRBaculovirus200ugQuote
MP340657BKRMammalian Cell200ugQuote

Protein Information

SpeciesBovine polyomavirus (BPyV)
UniProt IDP24851
Gene Name
Protein NameLarge T antigen
Region Expressed1-619
Expression Tag6xHis
Purity>90%
AA SequenceMELTSEEYEELRGLLGTPDIGNADTLKKAFLKACKVHHPDKGGNEEAMKRLLYLYNKAKI AASATTSQVPEYGTSQWEQWWEEFNQGFDEQDLHCDEELEPSDNEEENPAGSQAPGSQAT PPKKPRTSPDFPEVLKEYVSNALFTNRTYNCFIIFTTAEKGKELYPCIQAAYKCTFIALY MYNGDSVLYIITVGKHRVNAMENLCSKKCTVSFLQAKGVLKPQEAYNVCCTFELISQNIQ GGLPSSFFNPVQEEEKSVNWKLISEFACSIKCTDPLLLMALYLEFTTAPEACKVCDNPRR LEHRRHHTKDHTLNALLFQDSKTQKTICNQACDTVLAKRRLDMKTLTRNELLVQRWQGLF QEMEDLFGARGEEHLAHRMAAVMWLNALHPNMPDVIFNYIKMVVENKPKQRYLLLKGPVN CGKTTVAAGLIGLCGGAYLNINCPPERLAFELGMAIDQFTVVFEDVKGKKSSKSSLQTGI GFENLDNLRDHLDGAVPVNLERKHQNKVTQIFPPGIVTCNEYDIPLTVKIRMYQKVELLH NYNLYKSLKNTEEVGKKRYLQSGITWLLLLIYFRSVDDFTEKLQECVVKWKERIETEVGD MWLLTMKENIEQGKNILEK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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