Request QuoteCatalog Number: xP001117EJESize: 0.2-1mg

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Recombinant 3-ketoacyl-CoA thiolase (fadA)

Recombinant 3-ketoacyl-CoA thiolase (fadA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001117EJEYeast1mgQuote
EP001117EJEE. coli1mgQuote
BP001117EJEBaculovirus200ugQuote
MP001117EJEMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli O1:K1 / APEC
UniProt IDA1AI32
Gene NamefadA; Locus:Ecok1_38280; ORFs:APECO1_2612
Protein Name3-ketoacyl-CoA thiolase
Region Expressed1-387
Expression Tag6xHis
Purity>90%
AA SequenceMEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQ TLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEH MGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGISREMQDAFAARSHARAWAAT QSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVSGTVTAGTSSA LSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSAS DIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLM EHKDVQFGLATMCIGLGQGIATVFERV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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