Request QuoteCatalog Number: xP379456CWFSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP379456CWFYeast1mgQuote
EP379456CWFE. coli1mgQuote
BP379456CWFBaculovirus200ugQuote
MP379456CWFMammalian Cell200ugQuote

Protein Information

SpeciesCampylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176)
UniProt IDA1W1X2
Gene NameleuA; Locus:CJJ81176_0017
Protein Name2-isopropylmalate synthase
Region Expressed1-511
Expression Tag6xHis
Purity>90%
AA SequenceMKDNKIIIFDTTLRDGEQALGSSLGINQKLQIALALENLGVDVIEAGFPVSSQGDFKAVQ KIASKVKNSTICALSRALDKDIDMAYEALKVAKHFRIHTFIATSTLHMQDKLKKDFDEIL SMAKRAIIRARSYTDDVEFSCEDAGRTPIDNLCFMVENAIKAGAKTINIPDTVGYTLPSE FANIIKILFNKVPNIDKAIISVHCHNDLGVATGNSLSAILQGARQIECTINGLGERAGNC ALEEVVMAIKTRKDYLKGFYTDIKCENIFKTSKLVSAITNESIPSHKAIVGSNAFSHSSG IHQDGVLKNRQTYEIISPSAIGIHENRMLMTARSGRAMIKTCLENLGYDENTYNLDDVYE RFLRLADKKGQVYDYDLEALMFLSYENEEENEFVIEKLSVISGNIPTACVCMRIKEELKT EACTGNGPVEAVFNCIARITNLKPALKAYSINAKSSGVDAQGQVDVDLEFKGRKFHGKGI STDVIEASAQAFVSAYNAIYRSLKVEERKMA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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