Request QuoteCatalog Number: xP881179EZXSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP881179EZXYeast1mgQuote
EP881179EZXE. coli1mgQuote
BP881179EZXBaculovirus200ugQuote
MP881179EZXMammalian Cell200ugQuote

Protein Information

SpeciesPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
UniProt IDQ9HXK5
Gene NameleuA; Locus:PA3792
Protein Name2-isopropylmalate synthase
Region Expressed1-556
Expression Tag6xHis
Purity>90%
AA SequenceMSMLKDPSQKYRPFSAINLPDRTWPSKTITEVPIWCSSDLRDGNQSLIEPMDAAKKMRFF KTLVQVGLKQIEVAFPSASDTDFNFVRELIEGNHIPDDVTIQVLTQAREDLITRTFESLR GAKKAIVHVYNATAPSFRRIVFNQDKQGVVDIATNAAKLIKKLAAEQPETQWSFQYSPEI FSSTELEFSVEVCNAVIDVWQPTPDNKIILNLPATVECATPNVYADQIEWFGRHVDKRDS VIISLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTLALNMYTQGLHPQ LDFSDIDAVRKVVEECNQLPVHPRHPYVGDLVHTAFSGSHQDAIRKGFAQQKEDAIWEVP YLPIDPADIGRDYEAVIRVNSQSGKGGITFLLEQEYGISLPRRMQIEFSQVVQGETDRLG LEMTAQQIYSLLENEYLKATSPYVLASHRLQEENGTSAVDLEVSFDGEKQHWRGIGKGPL EALVAALPVKAEIMDYHEHAIGAGANAKAAAYIEIRLEGQRPLHGIGIDENITTASFRAL FSALNRAVTQAEAKAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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