Request QuoteCatalog Number: xP892313FHVSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP892313FHVYeast1mgQuote
EP892313FHVE. coli1mgQuote
BP892313FHVBaculovirus200ugQuote
MP892313FHVMammalian Cell200ugQuote

Protein Information

SpeciesPyrococcus abyssi (strain GE5 / Orsay)
UniProt IDQ9UZ08
Gene NameleuA; Locus:PYRAB13490; ORFs:PAB0890
Protein Name2-isopropylmalate synthase
Region Expressed1-486
Expression Tag6xHis
Purity>90%
AA SequenceMRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKL AVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFY ELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAAL EEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQ DGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFK DIADRKRWITDTDIEAIIQEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVAW FKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTD IIEASA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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