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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP886544DJAYeast1mgQuote
EP886544DJAE. coli1mgQuote
BP886544DJABaculovirus200ugQuote
MP886544DJAMammalian Cell200ugQuote

Protein Information

SpeciesDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
UniProt IDQ9RUA9
Gene NameleuA; Locus:DR_1482
Protein Name2-isopropylmalate synthase
Region Expressed1-522
Expression Tag6xHis
Purity>90%
AA SequenceMAMTQTENQTAPQQHIRIFDTTLRDGEQSPGVALNHAQKLEIAHTLARMGVDVIEAGFPI ASPGDLEGVSRIAREVRGPVITALARANRADIEAAAKGVEAAEKSRIHTFIATSPIHMQK KLNLEPDAVIERAVEAVRLARQYVDDVEFSAEDATRSEWDFLSRIFKAAVEAGATTINVP DTVGYTTPEEMRKLFAYLKAKLPAHVILSSHCHDDLGMAVANSIAAAEGGARQIECTING IGERAGNASLEEIVMAFHTRRDVYGYETGIRTRELYRASRLVSRLSGMPVQPNKAIVGDN AFAHESGIHQDGVIKARETYEIMNAELVGREAAVLVMGKHSGRAAFRKALTDLGYGDIPD DKVQHLFGRFKDLADRKGQIYAEDLRALVDARSDVPQTFSLEGFQITSGMNMMPVAFVRL HTPDGPVDATAHGDGPVEAAFQAISKITGISPTLESYRIQSVTRGDDALGEVSIHVRHGE TQLNGTGVATDIVEASARAWIRVMNMIVAGMGKNKAITQMTP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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