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Recombinant 2-isopropylmalate synthase 2, chloroplastic (IPMS2)

Recombinant 2-isopropylmalate synthase 2, chloroplastic (IPMS2) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP863202DOAYeast1mgQuote
EP863202DOAE. coli1mgQuote
BP863202DOABaculovirus200ugQuote
MP863202DOAMammalian Cell200ugQuote

Protein Information

SpeciesArabidopsis thaliana (Mouse-ear cress)
UniProt IDQ9C550
Gene NameIPMS2; aka: IMS1, MAML-3; Locus:At1g74040; ORFs:F2P9.9
Protein Name2-isopropylmalate synthase 2, chloroplastic
Region Expressed47-631
Expression Tag6xHis
Purity>90%
AA SequenceLTTAGKFRVSYSLSASSPLPPHAPRRRPNYIPNRISDPNYVRIFDTTLRDGEQSPGATLT SKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKTIAETVGNTVDENGYVPVICGLSRC NKKDIETAWEAVKYAKRPRIHTFIATSDIHLKYKLKKSKEEVIEIARNMVRFARSLGCED VEFSPEDAGRSEREYLYEILGEVIKAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIQ NVIISTHCQNDLGLSTANTLSGAHSGARQVEVTINGIGERAGNASLEEVVMAIKCRGDHV LGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEI MSPEEIGLERSNDAGIVLGKLSGRHALKDRLNELGYVLDDGQLSNLFWRFKAVAEQKKRV TDADLIALVSDEVFQPEAVWKLLDMQITCGTLGLSTSTVKLADSDGKEHVACSVGTGPVD AAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDNNYSSTNAVTGESVERTFS GTGAGMDIVVSSVKAYVGALNKMLGFKEHTSTLSKTPLETNEVPA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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