Request QuoteCatalog Number: xP886020CZASize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP886020CZAYeast1mgQuote
EP886020CZAE. coli1mgQuote
BP886020CZABaculovirus200ugQuote
MP886020CZAMammalian Cell200ugQuote

Protein Information

SpeciesCampylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
UniProt IDQ9PLV9
Gene NameleuA; Locus:Cj1719c
Protein Name2-isopropylmalate synthase
Region Expressed1-511
Expression Tag6xHis
Purity>90%
AA SequenceMKDNKIIIFDTTLRDGEQALGSSLGINQKLQIALALENLGVDVIEAGFPVSSQGDFKAVQ KIASKVKNSTICALSRALDKDIDMAYEALKVAKHFRIHTFIATSTLHMQDKLKKDFDEIL SMAKRAIIRARSYTDDVEFSCEDAGRTPIDNLCFMVENAIKAGAKTINIPDTVGYTLPSE FANIIKILFNKVPNIDKAIISVHCHNDLGMATGNSLSAILQGARQIECTINGLGERAGNC ALEEVVMAIKTRKDYLKGFYTDIKCENIFKTSKLVSAITNESIPSHKAIVGSNAFSHSSG IHQDGVLKNRQTYEIISPSAIGIHENRMLMTARSGRAMIKTCLENLGYDENTYNLDDVYE RFLRLADKKGQVYDYDLEALMFLSYESEEENEFVLEKLSVISGNIPTACVCMRIKEELKT EACTGNGPVEAVFNCIARITNLKPALKAYSINAKSSGVDAQGQVDVDLEFKGRKFHGKGL STDVIEASAQAFVSAYNAIYRSLKVEERKMA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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