Request QuoteCatalog Number: xP861358ESGSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP861358ESGYeast1mgQuote
EP861358ESGE. coli1mgQuote
BP861358ESGBaculovirus200ugQuote
MP861358ESGMammalian Cell200ugQuote

Protein Information

SpeciesPasteurella multocida (strain Pm70)
UniProt IDQ9CJN5
Gene NameleuA; Locus:PM1962
Protein Name2-isopropylmalate synthase
Region Expressed1-518
Expression Tag6xHis
Purity>90%
AA SequenceMADNIIIFDTTLRDGEQALKASLTVKEKLQIAFALERLGVDVMEVGFPISSAGDFESVQT IARHIKHSRVCALSRAVDKDIDAAAEALKVAEAFRIHTFIATSALHVEAKLRRTFEDVVD MAINAVKRARNYTDDVEFSCEDAGRTGVDNICRIVEAAIKAGATTVNIPDTVGYCLPYQY GDIIANVMNRVPNIDKAIISVHCHNDLGMATANSLTAVQNGARQIECTINGIGERAGNTA LEEVVMAIKTRQDMFNGLDTRINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGI HQDGMVKHKNTYEIMSPESIGLKKEKLNLTARSGRAAVKNHMSEMGYQESDYDLDKLYEA FLKLADKKGQVFDYDLEALAFIDMQQGDEDRLTLDVITSQCISHLPASAFVQVELDGKKM SQVSNGGNGPVDAVYNAILAITGLEMKMLNYNLTAKGEGAEALGQVDIVVEHEGRRFHGV GLATDIVESSARALIHAINAIYRSQKVADLKLHKIAGV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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