Request QuoteCatalog Number: xP871714MVNSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP871714MVNYeast1mgQuote
EP871714MVNE. coli1mgQuote
BP871714MVNBaculovirus200ugQuote
MP871714MVNMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium leprae
UniProt IDQ9CB76
Gene NameleuA; Locus:ML2324; ORFs:MLCB2407.26c
Protein Name2-isopropylmalate synthase
Region Expressed1-607
Expression Tag6xHis
Purity>90%
AA SequenceMASFSESLSQDPADAYKSAPSITKPMGPPSPGQPQWNPQRATSMPVFRYRPFAKEVEPIR LVDRTWPDRVIDCAPLWCAVDLRDGNQALIDPMSPICKRRMFDLLVRMGYKEIEVGFPSA SQTDFDFVREIITDGTIPDDVTIQVLTQCRPELIERTFEACENASRVIVHFYNSTSILQR RVVFRADQATVKAIATDGARKCVEEAFKYPGTHWRFEYSPESYTGTELEYAKQVCDAVGE VIQPTPDNPIIFNLPATVEMATPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAA ELGYHAGADRIEGCLFGNGERTGNVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNK LRVHERHPYGGDLVYTAFSGSHQDAINKGLDQMKIDADAADSDVDDILWQVPYLPIDPRD VGRTYEAVIRVNSQSGKGGVAYIMKADHGLELPRRLQIEFSRAIQKISEGEGGEITPTEM WDVFFEEYLSPVQPLERIKQRVNAAEEDGGSTSIAATVKINGEETEISGVGNGPLAAFID ALGHVGLQVAVLDYSEHAMNAGDDAQAAAYVEASVHGHTAWGVGIAPSITTASLRAVVSA VNRAMPR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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