Request QuoteCatalog Number: xP853850RCUSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP853850RCUYeast1mgQuote
EP853850RCUE. coli1mgQuote
BP853850RCUBaculovirus200ugQuote
MP853850RCUMammalian Cell200ugQuote

Protein Information

SpeciesRhizobium loti (strain MAFF303099) (Mesorhizobium loti)
UniProt IDQ98HN3
Gene NameleuA; Locus:mlr2792
Protein Name2-isopropylmalate synthase
Region Expressed1-558
Expression Tag6xHis
Purity>90%
AA SequenceMPDAARKYQPYPTVGLTDRTWPNKVIDKAPIWCSVDLRDGNQALIDPMGHERKARMFGLL LDMGFKEIEIGFPSASQTDFDFARWCIEEGNVPADVSLQVLVQCRPELITRTFEALKGAT NPIVHFYNSTSELQRRVVFEKDVGGIKRIATDAAKMITDMAAKAGGGYRFEYSPESFTGT ELEVALEICNAVTEIVRPKPDNKLIINLPSTVEMSTPNIYADRIEWMCRNLDNRENLIIS LHPHNDRGTGIATTELGLMAGADRVEGTLFGNGERTGNVDIVTLALNMYTQGVDPGIDCS DINRMKDVYEYSNQLKIPERHPYVGELVYTAFSGSHQDAINKGMKALKKANTSLWEVPYL PIDPADVGRSYEAIIRINSQSGKGGIAYVLQADYGLNLPRNLQIEFSQAIQAITDAEGKE VPAKRIHERFLETYVDQPGARLKFLDHHTYPDTAVKGRRVIEAVILDKGKEVTISGTGTG PIDGFVDALSRHVGVEMSVLDYSEHSMQRGSNASAISYVEMEYPGGKLFGAGINTNIVAA SLEAVTSAANRIVGRKAR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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