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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP858281DQOYeast1mgQuote
EP858281DQOE. coli1mgQuote
BP858281DQOBaculovirus200ugQuote
MP858281DQOMammalian Cell200ugQuote

Protein Information

SpeciesCaulobacter crescentus (strain ATCC 19089 / CB15)
UniProt IDQ9A823
Gene NameleuA; Locus:CC_1541
Protein Name2-isopropylmalate synthase
Region Expressed1-524
Expression Tag6xHis
Purity>90%
AA SequenceMTSVPAGAISKRDNVVVFDTTMRDGEQSPGASMSLEEKLELAKILEEMGVDVIEAGFPIA SNGDFEAVRQIAELITESTVCGLARAAAGDIDRCAEAVRRAKRGRIHTFISTSPVHMKYK LQMEPDAVLEAITRSVSHARNLVGDVEWSAEDATRTERDFLKRCVEAAIKAGATTINLPD TVGYSYPSEYGELFRDVITSVPGADKAIFSAHCHNDLGLAVANSIAAIEGGARQVEVAIN GIGERAGNAALEEIVMALRVRGDHLPYGTSVDPVHITRASRYVSAITGFPVQFNKAIVGK NAFAHESGIHQDGMLKNAETYEIMKPEDVGQGATNLVMGKHSGRHAFREKLKALGYELGQ NALNDAFGRFKELADKKKHVFDDDIVALVDDALARGSEKIRVSRLRVVAGTDGQSAELTL DIDGVASTAEATGDGPVDAVFNAIHKIVPHSAALRLFQVHAVTEGTDAQAQVSVRLEEDG RIATGAAADTDTLTASAKAYVNALNNLFARKEKSRPEAAIASGF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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