Request QuoteCatalog Number: xP881490BQSSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP881490BQSYeast1mgQuote
EP881490BQSE. coli1mgQuote
BP881490BQSBaculovirus200ugQuote
MP881490BQSMammalian Cell200ugQuote

Protein Information

SpeciesBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
UniProt IDQ9K8E8
Gene NameleuA; Locus:BH3058
Protein Name2-isopropylmalate synthase
Region Expressed1-515
Expression Tag6xHis
Purity>90%
AA SequenceMRKISVFDTTLRDGEQSAGVNLNFEEKMEIAKQLERLGVDIIEAGFPASSQGDFQSVKAI AETVKGSSVTGLARSVQKDIDAAWEALKSAEEPRIHLFIATSPIHMEHKLRMTPEQVIEK AVESVKYAASRFKHVQWSAEDASRSDFPFLAQIIEAVIQAGATVINLPDTVGYTTPQEIK RMFQYMKQNVPSIDKVSLSTHNHDDLGMAVANSLAAIQGGADQVECTINGIGERAGNASL EEIAVALNIRKDHYETETGLILKEIKRTSSLVSKLTGMVVPNNKAVVGANAFAHESGIHQ DGVLKNKQTYEIITPEMVGVSSNSMVLGKHSGRHAFKTKVQELGFTGTDEQLNNIFKAFK DLADKKKEITEDDLFALMTEQTVGGETNHYELQTLQVNYGSNLTNTATITMKLPSGEVAE EAATGSGSVEAIYNTLERLLDAPVKLLDYRIQSITGGRDALADVYVQMDYQGVVSSGRGT AHDVLEASAKAYLNAVNRTINRKKYAEVYGSKVEV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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