Request QuoteCatalog Number: xP842912DUBSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP842912DUBYeast1mgQuote
EP842912DUBE. coli1mgQuote
BP842912DUBBaculovirus200ugQuote
MP842912DUBMammalian Cell200ugQuote

Protein Information

SpeciesClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
UniProt IDQ97MC5
Gene NameleuA; Locus:CA_C0273
Protein Name2-isopropylmalate synthase
Region Expressed1-558
Expression Tag6xHis
Purity>90%
AA SequenceMLYNKYVKYPKVKLDNREWPDKQIEKAPIWCSVDLRDGNQALPKPMNVDEKIKMFKMLVD IGFKEIEIGFPSASETEYEFTRKLIEDKLIPEDVTIQVLTQAREHLVKKTFEALKGVKTA IVHVYNSTSELQRRVVFKKDKDEVKSLAIKGAQMVKKYSEYTEYSESKFVFEYSPESFTG TELDYALEVCEAVLNVWKPSKENKAIINLPSTVEMATPNIYADQIEWFCKKLLNRESVIL SLHTHNDRGTCTASSELGILAGADRVEGTLFGNGERTGNLDIMNMALNMYSQGVDPELEF SNLNDIVEAYEECTKMVVHERHPYAGKLVFTAFSGSHQDAIRKGLKSLKEGKNKHWEVPY LAVDPHDLGREYEEIIRINSQSGKGGTAYIMESDFGFILPKAMHPEFGKVIKKKSDELDC ELSPEQIFKFFKEEYLENRSPYYLKNYKIHSIQNIEEEKNTVDIEAVISVNGKDTSIEGV GNGPVDAFFNAMNNKKYNGCKFISYDEHALNIGSHSKAVAYVQIESQDKKYFGVGISDNI DTASINAIVSALNRSKLK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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